Other

Part:BBa_M31518:Design

Designed by: Sophie Wong   Group: BE.109 Instructors BE.109 2006 MIT 20.109 Spring07 MIT 20.109 Fall07   (2007-02-27)


Refactored M13K07 from Hpa1 cut site to BamH1 cut site


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1523
    Illegal EcoRI site found at 1534
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1523
    Illegal EcoRI site found at 1534
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1523
    Illegal EcoRI site found at 1534
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1523
    Illegal EcoRI site found at 1534
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1523
    Illegal EcoRI site found at 1534
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Which restriction enzymes to include, which bases to mutate to eliminate overlaps of DNA elements.


Source

M13K07 genome

References